GreenLight is dedicated to bringing our Cell-Free process for the production of RNA to market to address global needs and threats. The team at GreenLight is composed of world leaders in diverse fields all working towards one goal. A dynamic and fast-paced environment is cultivated with employees not only aligned with GreenLight's mission, but also passionate about the technology and science. We are always seeking passionate and talented people that are ready to lead the coming RNA revolution.
GreenLight is an equal opportunity employer. We pride ourselves on the diversity of our workforce. We believe in a culture of transparency, integrity, gender equality and inclusion.
Scientist I / II – Pathway & Process Development
GreenLight Biosciences is seeking an exceptional, highly-motivated metabolic or biochemical engineer to work on cutting edge cell-free bioprocessing technology. The successful candidate will support research on the discovery, engineering, and optimization of biochemical pathways & processes in the context of several milestone-driven projects. Strong technical, organizational, and communication skills, as well as the ability to develop creative solutions to challenging problems, are essential.
- Design, execute & analyze experiments at different scales (ranging from microliters to tens of liters) for pathway & process testing, debottlenecking & optimization
- Research biological pathways and associated enzymes for the production of target molecules
- Perform molecular biology techniques such as PCR, DNA subcloning, in vitro transcription, cell transformation, cell culture, and other more advanced methods in support of research projects
- Collaborate with the analytical team to identify & develop analytical methods to investigate the cell-free reaction system
- Collaborate with the downstream processing team in developing processes for improved recovery of pathway enzymes & products of interest
- Work collaboratively with other team members to accomplish project goals and milestones
- Use creative problem-solving skills to independently troubleshoot experimental challenges
- Analyze, summarize, and present experimental results to team members and management
- Contribute to technical discussions related to the development and application of GreenLight’s proprietary bioproduction technology
- Continuously contribute to the company’s intellectual property portfolio and routinely track and document results in laboratory notebooks and databases
- Ph.D. in chemical engineering, bioengineering, molecular biology, biochemistry, or equivalent
- 0-3 years of experience in the biotechnology industry
- Fundamental understanding of principles of biochemical engineering, material & energy balances & metabolic networks is required
- Strong background in molecular biology, microbial physiology, and biochemical pathway engineering with expertise on cutting-edge technologies for pathway/organism manipulation
- Experience in process development, statistical design of experiments & modeling is highly desired
- Familiarity with techniques for metabolite, protein & nucleic acid isolation, detection & quantitation as well as development of enzyme assays is a major plus
- Must be highly-motivated with exceptional communication, leadership, and teamwork skills
Scientist I/II – Nucleic Acid Biochemistry
GreenLight Biosciences is seeking an exceptional and highly motivated analytical scientist to help advance the company’s RNA platform technology. The successful candidate will support R&D efforts within the company by characterizing RNA products with respect to structure, sequence, and modifications. A deep technical background in analytical chemistry, molecular biology, and assay development is required. Strong organizational and communication skills, creative problem-solving, and flexibility are essential.
- Characterize the structure, sequence, and modifications of RNA molecules using state-of-the-art techniques, including HPLC, LC-MS/MS, capillary electrophoresis, next-generation sequencing, etc.
- Purify RNA products using state-of-the art techniques, such as reverse-phase chromatography, ion exchange chromatography, size exclusion chromatography, solid-phase extraction, etc.
- Evaluate purity of RNA samples for nucleic acid, protein, and other contaminants
- Design and perform standard molecular biology experiments (DNA cloning, nuclease digestion, PCR, (q)RT-PCR, agarose gel electrophoresis, etc.)
- Develop novel analytical methods where necessary
- Work independently and in collaboration with multidisciplinary project teams, as well as the Analytical Chemistry and Metabolic Engineering groups
- Maintain diligent and organized records
- Clearly communicate results and recommendations to colleagues, managers, and senior management, assisting in project decision-making
- Ph.D. in Biochemistry, Molecular Biology, or related discipline with at least 3 years of industrial experience or 5 years of academic experience, specializing in the RNA field. Candidates with a M.S. and at least 8 years of industrial experience in the RNA field will also be considered.
- Experience characterizing nucleic acid modifications, including nucleoside and post-transcriptional modifications (e.g. 5’-cap structures, polyadenylation, etc.) is required
- Experience characterizing primary and secondary structures of nucleic acids is required
- Experience developing analytical chemistry methods (e.g. HPLC and LC-MS) is required
- Experience with standard molecular biology techniques is required
- Experience with next-generation approaches to RNA sequencing is desirable
- Experience translating bench scale experiments to high throughput workflows is desirable
- Strong organization, communication, and interpersonal skills are required, with the ability to work both independently and in a collaborative work environment